Alterations of phospholipid and triglyceride metabolism in fatty liver caused by pyridoxine deficiency in rats.

When weanling rats were given 70% casein diet deficient in pyridoxine, they showed marked accumulation of liver lipid consisting mainly of triglyceride (TG) and cholesterol ester (CE). The serum concentrations of phospholipid (PL) and cholesterol (CH) in pyridoxine deficient rats were significantly lower than those in pair-fed controls, but their serum levels of TG and free fatty acid (FFA) were not significantly different from those of controls. A significant inverse relation was shown between the concentration of liver TG and PL of pyridoxine-deficient rats. Measurement of incorporation of radioactivity from [1-14C]acetate into lipid in vivo showed that lipogenesis in the liver of pyridoxine-deficient rats was depressed, although the acetate pool was significantly higher than that in pair-fed controls. The incorporations of radioactivity from [1 -14C]acetate into liver TG and PL in vivo were also lower in pyridoxine